Cancer-associated fibroblasts mediate cancer progression and remodel the tumouroid stroma.

ABSTRACT:

Background

Cancer-associated fibroblasts (CAFs) are highly differentiated and heterogeneous cancer-stromal cells that promote tumour growth, angiogenesis and matrix remodelling.

Methods

We utilised an adapted version of a previously developed 3D in vitro model of colorectal cancer, composed of a cancer mass and the surrounding stromal compartment. We compared cancer invasion with an acellular stromal surround, a "healthy" or normal cellular stroma and a cancerous stroma. For the cancerous stroma, we incorporated six patient-derived CAF samples to study their differential effects on cancer growth, vascular network formation and remodelling.

Results

CAFs enhanced the distance and surface area of the invasive cancer mass whilst inhibiting vascular-like network formation.

SUBMITTER: Pape J 

PROVIDER: S-EPMC7524802 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature