Nonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles.

ABSTRACT: This study aims to suggest a simple migratory cell monitoring method in the Transwell system by utilizing retroreflective Janus microparticles (RJPs) as an optical probe. The RJP could be internalized on cells without compromising the cell viability and can be registered as bright spots within the cell body by inducing retroreflection from nonspectroscopic light sources. Conventional optical probes (e.g., fluorophores, chromogens, and nanoparticles) have been extensively studied and applied across diverse platforms (e.g., Boyden chamber, wound closing, and microfluidic chips) for understanding in vitro kinetic cell behavior. However, the complexities of running such platforms and setting up analytical instruments are limiting. In this regard, we aimed to demonstrate a modified Transwell mi

SUBMITTER: Kim KR 

PROVIDER: S-EPMC7528338 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature