Dataset Information

Association of vitamin D₃ and its metabolites in patients with and without type 2 diabetes and their relationship to diabetes complications.

ABSTRACT:

Background

Epidemiological studies have suggested that vitamin D deficiency is associated with the development of type 2 diabetes (T2DM) and is related to diabetes complications. This study was undertaken to determine the relationship between diabetes complications and cardiovascular risk factors with vitamin D₃ and its metabolites: 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃), 25-hydroxyvitamin D₃ (25(OH)D₃), 24,25-dihydroxyvitamin D₃ (24,25(OH)₂D₃); and 25-hydroxy-3epi-vitamin D₃ (3epi25(OH)D₃).

Methods

750 Qatari subjects, 460 (61.3%) with and 290 (38.7%) without T2DM, who were not taking vitamin D₃ supplements, participated in this cross-sectional, observational study. Plasma concentrations of vitamin D₃ and its metabolites were measured by liquid chromatography tandem mass spectrometry analysis.

Results

T2DM subjects had lower concentrations of all vitamin D₃ metabolites (p < 0.001) except 3epi25(OH)D₃ (p < 0.071). Males had higher concentrations of all vitamin D₃ metabolites (p < 0.001). In the T2DM subjects, lower 25(OH)D₃ was associated with retinopathy (p < 0.03) and dyslipidemia (p < 0.04), but not neuropathy or vascular complications; lower 1,25(OH)₂D₃ was associated with hypertension (p < 0.009), dyslipidemia (p < 0.003) and retinopathy (p < 0.006), and coronary artery disease (p < 0.012), but not neuropathy; lower 24,25(OH)₂D₃ concentrations were associated with dyslipidemia alone (p < 0.019); 3epi25(OH)D₃ associated with diabetic neuropathy alone (p < 0.029). In nondiabetics, 25(OH)D₃, 1,25(OH)₂D₃ and 24,25(OH)₂D₃ were associated with dyslipidemia (p < 0.001, p < 0.001, p < 0.015, respectively) and lower 1,25(OH)₂D₃ was associated with hypertension (p < 0.001). Spearman's correlation showed 1,25(OH)₂D₃ to be negatively correlated to age and diabetes duration.

Conclusions

Different diabetes complications were associated with differing vitamin D parameters, with diabetic retinopathy related to lower 25(OH)D₃ and 1,25(OH)₂D₃ levels, hypertension significantly associated with lower 1,25(OH)₂D₃, while dyslipidemia was associated with lower 25(OH)D₃, 1,25(OH)₂D₃ and 24,25(OH)₂D_3. While 25(OH)D metabolites were lower in females, there was not an exaggeration in complications.

SUBMITTER: Butler AE

PROVIDER: S-EPMC7534081 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Association of vitamin D3 and its metabolites in patients with and without type 2 diabetes and their relationship to diabetes complications.

Butler Alexandra E AE Dargham Soha R SR Latif Aishah A Mokhtar Haira R HR Robay Amal A Chidiac Omar M OM Jayyousi Amin A Al Suwaidi Jassim J Crystal Ronald G RG Abi Khalil Charbel C Atkin Stephen L SL

Therapeutic advances in chronic disease 20200926

<h4>Background</h4>Epidemiological studies have suggested that vitamin D deficiency is associated with the development of type 2 diabetes (T2DM) and is related to diabetes complications. This study was undertaken to determine the relationship between diabetes complications and cardiovascular risk factors with vitamin D3 and its metabolites: 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), 25-hydroxyvitamin D3 (25(OH)D3), 24,25-dihydroxyvit ...[more]

PMID: 33062234

Similar Datasets

Project description:BackgroundVitamin D deficiency is diagnosed by total serum 25-hydroxyvitamin D (25(OH)D) concentration and is associated with poor health and increased mortality; however, some populations have low 25(OH) D concentrations without manifestations of vitamin D deficiency. The Vitamin D Metabolite Ratio (VMR) has been suggested as a superior indicator of vitamin D status. Therefore, VMR was determined in a population with type 2 diabetes at high risk for vitamin D deficiency and correlated with diabetic complications.Research design and methodsFour hundred sisty patients with type 2 diabetes (T2D) were recruited, all were vitamin D3 supplement naive. Plasma concentration of 25-hydroxyvitamin D3 (25(OH)D3) and its metabolites 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) and its epimer, 3-epi-25-hydroxyvitamin D3 (3-epi-25(OH)D3), were measured by LC-MS/MS analysis. VMR-1 was calculated as a ratio of 24,25(OH)2D3:25(OH)D3; VMR-2 as a ratio of 1,25(OH)2D3:25(OH)D3; VMR-3 was calculated as a ratio of 3-epi-25(OH)D3: 25(OH)D3. RESULTS: An association means that there were significant differences between the ratios found for those with versus those without the various diabetic complications studied. VMR-1 was associated with diabetic retinopathy (p = 0.001) and peripheral artery disease (p = 0.012); VMR-2 associated with hypertension (p < 0.001), dyslipidemia (p < 0.001), diabetic retinopathy (p < 0.001), diabetic neuropathy (p < 0.001), coronary artery disease (p = 0.001) and stroke (p < 0.05). VMR-3 associated with hypertension (p < 0.05), dyslipidemia (p < 0.001) and coronary artery disease (p < 0.05).ConclusionsIn this cross sectional study, whilst not causal, VMR-2 was shown to be the superior predictor of diabetic and cardiovascular complications though not demonstrative of causality in this cross-sectional study population over VMR-1, VMR-3 and the individual vitamin D concentration measurements; VMR-2 associated with both microvascular and cardiovascular indices and therefore may have utility in predicting the development of diabetic complications.