Isatis root polysaccharide promotes maturation and secretory function of monocyte-derived dendritic cells.

ABSTRACT:

Background

Pseudorabies virus (PRV) is an animal virus that is globally responsible for the high economic losses in the swine industry. Isatis root is a traditional Chinese medicinal herb that possesses immune-enhancing and antiviral properties. However, the molecular mechanisms underlying the effects of the active component of the isatis root polysaccharide (IRPS) extract on immature dendritic cells remain elusive.

Methods

In this study, we investigated the molecular changes in primary porcine peripheral blood monocyte-derived dendritic cells (MoDCs) during PRV infection, using enzyme-linked immunosorbent assay (ELISA) and quantitative reverse transcription-polymerase chain reaction. Additionally, we studied the effect of IRPS on PRV-infected DCs.

Results

The results

SUBMITTER: Wang X 

PROVIDER: S-EPMC7542110 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature