MiR-34 regulates larval growth and wing morphogenesis by directly modulating ecdysone signalling and cuticle protein in Bombyx mori.

ABSTRACT: microRNAs (miRNA) are small non-coding RNAs that modulate the myriad biological activities by targeting genes, and many studies showed that miRNAs played a pivotal role in insect development. Here, we find that Bm-miRNA (miR-34) controls larval growth and wing morphology by targeting BmE74 and BmCPG4. Overexpression of miR-34 in the whole body caused a smaller body size, partially displays deformed wings and venation defects in adults. Ablation of miR-34 by transgenic CRISPR/Cas9 technology resulted in a severe developmental delay during the larval stage. Moreover, we confirmed that miR-34 directly targeted BmE74 and BmCPG4 by using a dual luciferase reporter assay in HEK293T cells. Remarkably, loss-of-function of BmCPG4 caused wing defects, which was sim

SUBMITTER: Liu Z 

PROVIDER: S-EPMC7549633 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature