Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages.

ABSTRACT:

Objectives

To investigate the relevance of multicopy plasmids in antimicrobial resistance and assess their mobilization mediated by phage particles.

Methods

Several databases with complete sequences of plasmids and annotated genes were analysed. The 16S methyltransferase gene armA conferring high-level aminoglycoside resistance was used as a marker in eight different plasmids, from different incompatibility groups, and with differing sizes and plasmid copy numbers. All plasmids were transformed into Escherichia coli bearing one of four different lysogenic phages. Upon induction, encapsidation of armA in phage particles was evaluated using qRT-PCR and Southern blotting.

Results

Multicopy plasmids carry a vast set of emerging clinically important antimicrobial resistanc

SUBMITTER: Rodriguez-Rubio L 

PROVIDER: S-EPMC7566468 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature