Non-Transcriptional and Translational Function of Canonical NF-κB Signaling in Activating ERK1/2 in IL-1β-Induced COX-2 Expression in Synovial Fibroblasts.

ABSTRACT: The pro-inflammatory cytokine interleukin 1β (IL-1β) induces the synthesis of prostaglandin E₂ by upregulating cyclooxygenase-2 (COX-2) in the synovial tissue of individuals with autoimmune diseases, such as rheumatoid arthritis (RA). IL-1β-mediated stimulation of NF-κB and MAPK signaling is important for the pathogenesis of RA; however, crosstalk(s) between NF-κB and MAPK signaling remains to be understood. In this study, we established a model for IL-1β-induced synovitis and investigated the role of NF-κB and MAPK signaling in synovitis. We observed an increase in the mRNA and protein levels of COX-2 and prostaglandin E₂ release in cells treated with IL-1β. NF-κB and ERK1/2 inhibitors significantly reduc

SUBMITTER: Nakano R 

PROVIDER: S-EPMC7576893 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature