Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTP/MPP⁺-Induced Toxicity via Progesterone Receptor Signaling.

ABSTRACT:

Background

Progesterone receptor (PR) modulates neuroprotective and regenerative responses in Parkinson's disease and related neurological diseases.

Objectives

The present study was designed to determine whether botanical drug puerarin could exhibit neuroprotective and neurorestorative activities via PR signaling.

Methods

The neuroprotective and neurotrophic activities of puerarin were investigated in MPTP-lesioned mice and MPP⁺-challenged primary rat midbrain neurons. Rotarod performance test and tail suspension test were used to assess motor functions. Tyrosine hydroxylase (TH) and PR were determined by immunostaining, Western blotting, and luciferase reporter assays. Neurite outgrowth was assessed by fluorescence staining and immunostaining.

Results</

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7586160 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature