Optimizing imaging speed and excitation intensity for single-molecule localization microscopy.
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ABSTRACT:
SUBMITTER: Diekmann R
PROVIDER: S-EPMC7610360 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Publications
Optimizing imaging speed and excitation intensity for single-molecule localization microscopy.
Diekmann Robin R Kahnwald Maurice M Schoenit Andreas A Deschamps Joran J Matti Ulf U Ries Jonas J
Nature methods 20200817 9
High laser powers are common practice in single-molecule localization microscopy to speed up data acquisition. Here we systematically quantified how excitation intensity influences localization precision and labeling density, the two main factors determining data quality. We found a strong trade-off between imaging speed and quality and present optimized imaging protocols for high-throughput, multicolor and three-dimensional single-molecule localization microscopy with greatly improved resolutio ...[more]
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