Ultra-high-throughput single-cell RNA sequencing and perturbation screening with combinatorial fluidic indexing.

ABSTRACT: Cell atlas projects and high-throughput perturbation screens require single-cell sequencing at a scale that is challenging with current technology. To enable cost-effective single-cell sequencing for millions of individual cells, we developed 'single-cell combinatorial fluidic indexing' (scifi). The scifi-RNA-seq assay combines one-step combinatorial preindexing of entire transcriptomes inside permeabilized cells with subsequent single-cell RNA-seq using microfluidics. Preindexing allows us to load several cells per droplet and computationally demultiplex their individual expression profiles. Thereby, scifi-RNA-seq massively increases the throughput of droplet-based single-cell RNA-seq, and provides a straightforward way of multiplexing thousands of samples in a single experiment. Compared

SUBMITTER: Datlinger P 

PROVIDER: S-EPMC7612019 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature