Species-level, metagenomic and proteomic analysis of microbe-immune interactions in severe asthma.

ABSTRACT:

Background

The airway microbiome in severe asthma has not been characterised at species-level by metagenomic sequencing, nor have the relationships between specific species and mucosal immune responses in 'type-2 low', neutrophilic asthma been defined. We performed an integrated species-level metagenomic data with inflammatory mediators to characterise prevalence of dominant potentially pathogenic organisms and host immune responses.

Methods

Sputum and nasal lavage samples were analysed using long-read metagenomic sequencing with Nanopore and qPCR in two cross-sectional adult severe asthma cohorts, Wessex (n = 66) and Oxford (n = 30). We integrated species-level data with clinical parameters and 39 selected airway proteins measured by immunoassay and O-link.

Results

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SUBMITTER: Jabeen MF 

PROVIDER: S-EPMC7617782 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature