Aberrant NSUN2-mediated m⁵C modification of H19 lncRNA is associated with poor differentiation of hepatocellular carcinoma.

ABSTRACT: RNA methylation is an important epigenetic modification. Recent studies on RNA methylation mainly focus on the m⁶A modification of mRNA, but very little is known about the m⁵C modification. NSUN2 is an RNA methyltransferase responsible for the m⁵C modification of multiple RNAs. In this study, we knocked down the NSUN2 gene in HepG2 cells by CRISPR/Cas9 technology and performed high-throughput RNA-BisSeq. An important tumor-related lncRNA H19 was identified to be targeted by NSUN2. Studies have shown that the expression of H19 lncRNA is abnormally elevated and has a carcinogenic effect in many types of tumors. Our results demonstrated that m⁵C modification of H19 lncRNA can increase its stability. Interestingly, m⁵C-modified H19 lncRNA

SUBMITTER: Sun Z 

PROVIDER: S-EPMC7644462 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature