N-Terminomics for the Identification of In Vitro Substrates and Cleavage Site Specificity of the SARS-CoV-2 Main Protease.

ABSTRACT: The genome of coronaviruses, including SARS-CoV-2, encodes for two proteases, a papain like (PL^pro ) protease and the so-called main protease (M^pro ), a chymotrypsin-like cysteine protease, also named 3CL^pro or non-structural protein 5 (nsp5). M^pro is activated by autoproteolysis and is the main protease responsible for cutting the viral polyprotein into functional units. Aside from this, it is described that M^pro proteases are also capable of processing host proteins, including those involved in the host innate immune response. To identify substrates of the three main proteases from SARS-CoV, SARS-CoV-2, and hCoV-NL63 coronviruses, an LC-MS based N-terminomics in vitro analysis is performed using recombinantly expressed proteases and

SUBMITTER: Koudelka T 

PROVIDER: S-EPMC7645863 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature