Effect of acute noise trauma on the gene expression profile of the hippocampus.

ABSTRACT: BACKGROUND:This study aimed to investigate the changes in the expression of hippocampal genes upon acute noise exposure. METHODS:Three-week-old Sprague-Dawley rats were assigned to control (n?=?15) and noise (n?=?15) groups. White noise (2-20 kHz, 115 dB sound pressure level [SPL]) was delivered for 4 h per day for 3 days to the noise group. All rats were sacrificed on the last day of noise exposure, and gene expression in the hippocampus was analyzed using a microarray. Pathway analyses were conducted for genes that showed differential expression???1.5-fold and P???0.05 compared to the control group. The genes included in the putative pathways were measured using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). RESULTS:Thirty-eight upregulated genes and 81 downregulated genes were identified. The pathway analyses revealed that upregulated genes were involved in the cellular responses to external stimuli and immune system pathways. qRT-PCR confirmed the upregulation of the involved genes. The downregulated genes were involved in neuronal systems and synapse-related pathways, and qRT-PCR confirmed the downregulation of the involved genes. CONCLUSIONS:Acute noise exposure upregulated the expression of immune-related genes and downregulated the expression of neurotransmission-related genes in the hippocampus.

SUBMITTER: Lee CH 

PROVIDER: S-EPMC7648995 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature