Predictive design of sigma factor-specific promoters.

ABSTRACT: To engineer synthetic gene circuits, molecular building blocks are developed which can modulate gene expression without interference, mutually or with the host's cell machinery. As the complexity of gene circuits increases, automated design tools and tailored building blocks to ensure perfect tuning of all components in the network are required. Despite the efforts to develop prediction tools that allow forward engineering of promoter transcription initiation frequency (TIF), such a tool is still lacking. Here, we use promoter libraries of E. coli sigma factor 70 (σ⁷⁰)- and B. subtilis σ^B-, σ^F- and σ^W-dependent promoters to construct prediction models, capable of both predicting promoter TIF and orthogonality of the σ-specific promoters. This is

SUBMITTER: Van Brempt M 

PROVIDER: S-EPMC7670410 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature