An empirical bayesian approach for testing gene expression fold change and its application in detecting global dosage effects.

ABSTRACT: We are motivated by biological studies intended to understand global gene expression fold change. Biologists have generally adopted a fixed cutoff to determine the significance of fold changes in gene expression studies (e.g. by using an observed fold change equal to two as a fixed threshold). Scientists can also use a t-test or a modified differential expression test to assess the significance of fold changes. However, these methods either fail to take advantage of the high dimensionality of gene expression data or fail to test fold change directly. Our research develops a new empirical Bayesian approach to substantially improve the power and accuracy of fold-change detection. Specifically, we more accurately estimate gene-wise error variation in the log of fold change. We then ado

SUBMITTER: Guo Z 

PROVIDER: S-EPMC7671412 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature