Ontology highlight
ABSTRACT: Background and objectives
Outbred mice are widely used in toxicology, pharmacology, and fundamental biomedical research. However, there have been no reports of in vitro culture systems for spermatogonial stem cells (SSCs) derived from these mice.Methods
As a step towards constructing a non-cellular niche supporting the in vitro maintenance of outbred mouse SSC self-renewal, we systematically investigated the types of integrin heterodimers that are expressed transcriptionally, translationally, and functionally in SSCs derived from Imprinting Control Region (ICR) mice.Results
Among the genes encoding 25 integrin subunits, integrin α1, α5, α6, α9, αV, and αE, and integrin β1 and β5 had significantly higher transcriptional levels than the other subunits. Furthermore, at the translational level, integrin α5, α6, α9, αV, αE, and β1 were localized on the surface of SSCs, but integrin α1 and β5 not. Moreover, significantly stronger translational expression than integrin α9 and αE was observed in integrin α5, α6, αV, and β1. SSCs showed significantly increased adhesion to fibronectin, laminin, tenascin C and vitronectin, and functional blocking of integrin α5β1, α6β1, α9β1 or αVβ1 significantly inhibited adhesion to these molecules.Conclusions
We confirmed that integrin α5β1, α6β1, α9β1 and αVβ1 actively function on the surface of undifferentiated SSCs derived from outbred ICR mice.
SUBMITTER: Park HJ
PROVIDER: S-EPMC7691863 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Park Hye Jin HJ Yun Jung Im JI Kim Minseok M Choi Kimyung K Lee Eunsong E Lee Seung Tae ST
International journal of stem cells 20201101 3
<h4>Background and objectives</h4>Outbred mice are widely used in toxicology, pharmacology, and fundamental biomedical research. However, there have been no reports of <i>in vitro</i> culture systems for spermatogonial stem cells (SSCs) derived from these mice.<h4>Methods</h4>As a step towards constructing a non-cellular niche supporting the <i>in vitro</i> maintenance of outbred mouse SSC self-renewal, we systematically investigated the types of integrin heterodimers that are expressed transcri ...[more]