Screening of Integrin Heterodimers Expressed Functionally on the Undifferentiated Spermatogonial Stem Cells in the Outbred ICR Mice.

ABSTRACT:

Background and objectives

Outbred mice are widely used in toxicology, pharmacology, and fundamental biomedical research. However, there have been no reports of in vitro culture systems for spermatogonial stem cells (SSCs) derived from these mice.

Methods

As a step towards constructing a non-cellular niche supporting the in vitro maintenance of outbred mouse SSC self-renewal, we systematically investigated the types of integrin heterodimers that are expressed transcriptionally, translationally, and functionally in SSCs derived from Imprinting Control Region (ICR) mice.

Results

Among the genes encoding 25 integrin subunits, integrin α₁, α₅, α₆, α₉, α_V, and α

SUBMITTER: Park HJ 

PROVIDER: S-EPMC7691863 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature