Evaluation of the upper airway microbiome and immune response with nasal epithelial lining fluid absorption and nasal washes.

ABSTRACT: Despite being commonly used to collect upper airway epithelial lining fluid, nasal washes are poorly reproducible, not suitable for serial sampling, and limited by a dilution effect. In contrast, nasal filters lack these limitations and are an attractive alternative. To examine whether nasal filters are superior to nasal washes as a sampling method for the characterization of the upper airway microbiome and immune response, we collected paired nasal filters and washes from a group of 40 healthy children and adults. To characterize the upper airway microbiome, we used 16S ribosomal RNA and shotgun metagenomic sequencing. To characterize the immune response, we measured total protein using a BCA assay and 53 immune mediators using multiplex magnetic bead-based assays. We conducted statistica

SUBMITTER: Shilts MH 

PROVIDER: S-EPMC7692476 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature