Strategy for identification of cis-dihydrodiendiol-degrading dehydrogenases in E. coli BW25113.

ABSTRACT: cis-Dihydrodiendiols are valuable compounds, finding multiple application as chiral synthons in organic chemistry. The biotechnological route for the generation of cis-dihydrodiendiols involves the dihydroxylation of aromatic compounds, catalyzed by Rieske non-heme iron dioxygenases. To date, numerous examples of recombinant E. coli, harboring such dioxygenases, can be found in the literature. Nevertheless, there is only a minor number of publications, addressing the E. coli catalyzed degradation of cis-dihydrodiendiols into catechols via dehydrogenases. Identification and elimination of such dehydrogenase catalyzed degradation is key for the establishment of enhanced recombinant E. coli platforms pursuing the production of cis-dihydr

SUBMITTER: Wissner JL 

PROVIDER: S-EPMC7704418 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature