Unknown

Dataset Information

0

Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network.


ABSTRACT: Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system of ordinary differential equations describing temporal signal network dynamics. Bifurcation analysis and numerical simulations predict a strong dependence of oscillatory system dynamics on the concentration of GEF-H1, an Lbc-type RhoGEF, which mediates the positive feedback amplification of Rho activity. This prediction is confirmed experimentally via optogenetic tuning of the effective GEF-H1 concentration in individual living cells. Numerical simulations show that pulse amplitude is most sensitive to external inputs into the myosin component at low GEF-H1 concentrations and that the spatial pulse width is dependent on GEF-H1 diffusion. Our study offers a theoretical framework to explain the emergence of local cell contraction pulses and their modulation by biochemical and mechanical signals.

SUBMITTER: Kamps D 

PROVIDER: S-EPMC7710677 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5716289 | biostudies-literature
| S-EPMC8595684 | biostudies-literature
| S-EPMC5566799 | biostudies-other
| S-EPMC2190572 | biostudies-literature
| S-EPMC7718400 | biostudies-literature
| S-EPMC6344499 | biostudies-literature
| S-EPMC7648432 | biostudies-literature
| S-EPMC9603463 | biostudies-literature
| S-EPMC2682016 | biostudies-literature
2011-10-07 | E-GEOD-32703 | biostudies-arrayexpress