Condensation of Rubisco into a proto-pyrenoid in higher plant chloroplasts.

ABSTRACT: Photosynthetic CO₂ fixation in plants is limited by the inefficiency of the CO₂-assimilating enzyme Rubisco. In most eukaryotic algae, Rubisco aggregates within a microcompartment known as the pyrenoid, in association with a CO₂-concentrating mechanism that improves photosynthetic operating efficiency under conditions of low inorganic carbon. Recent work has shown that the pyrenoid matrix is a phase-separated, liquid-like condensate. In the alga Chlamydomonas reinhardtii, condensation is mediated by two components: Rubisco and the linker protein EPYC1 (Essential Pyrenoid Component 1). Here, we show that expression of mature EPYC1 and a plant-algal hybrid Rubisco leads to spontaneous condensation of Rubisco into a single phase-separated compartment in Arabid

SUBMITTER: Atkinson N 

PROVIDER: S-EPMC7726157 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature