Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells.

ABSTRACT: Light-inducible dimerization protein modules enable precise temporal and spatial control of biological processes in non-invasive fashion. Among them, Magnets are small modules engineered from the Neurospora crassa photoreceptor Vivid by orthogonalizing the homodimerization interface into complementary heterodimers. Both Magnets components, which are well-tolerated as protein fusion partners, are photoreceptors requiring simultaneous photoactivation to interact, enabling high spatiotemporal confinement of dimerization with a single excitation wavelength. However, Magnets require concatemerization for efficient responses and cell preincubation at 28°C to be functional. Here we overcome these limitations by engineering an optimized Magnets pair requiring neither concatemerization nor l

SUBMITTER: Benedetti L 

PROVIDER: S-EPMC7735757 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature