Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement.

ABSTRACT: Sciatic nerve crush injury triggers sterile inflammation within the distal nerve and axotomized dorsal root ganglia (DRGs). Granulocytes and pro-inflammatory Ly6C^high monocytes infiltrate the nerve first and rapidly give way to Ly6C^negative inflammation-resolving macrophages. In axotomized DRGs, few hematogenous leukocytes are detected and resident macrophages acquire a ramified morphology. Single-cell RNA-sequencing of injured sciatic nerve identifies five macrophage subpopulations, repair Schwann cells, and mesenchymal precursor cells. Macrophages at the nerve crush site are molecularly distinct from macrophages associated with Wallerian degeneration. In the injured nerve, macrophages 'eat' apoptotic leukocytes, a process called efferocytosis, and thereby promote an

SUBMITTER: Kalinski AL 

PROVIDER: S-EPMC7735761 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature