High-density transposon libraries utilising outward-oriented promoters identify mechanisms of action and resistance to antimicrobials.

ABSTRACT: The use of bacterial transposon mutant libraries in phenotypic screens is a well-established technique for determining which genes are essential or advantageous for growth in conditions of interest. Standard, inactivating, transposon libraries cannot give direct information about genes whose over-expression gives a selective advantage. We report the development of a system wherein outward-oriented promoters are included in mini-transposons, generation of transposon mutant libraries in Escherichia coli and Pseudomonas aeruginosa and their use to probe genes important for growth under selection with the antimicrobial fosfomycin, and a recently-developed leucyl-tRNA synthase inhibitor. In addition to the identification of known mechanisms of action and resistance, we identify the carbon-phosp

SUBMITTER: Coward C 

PROVIDER: S-EPMC7735965 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature