Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans.

ABSTRACT: To characterize the molecular mechanisms underlying life-stage transitions in Phytophthora infestans, we initiated a chemical genetics approach by screening for a stage-specific inhibitor of morphological development from microbial culture extracts prepared mostly from actinomycetes from soil in Japan. Of the more than 700 extracts, one consistently inhibited Ph. infestans cyst germination. Purification and identification of the active compound by ESI-MS, ¹H-NMR, and ¹³C-NMR identified β-rubromycin as the inhibitor of cyst germination (IC₅₀ = 19.8 μg/L); β-rubromycin did not inhibit growth on rye media, sporangium formation, zoospore release, cyst formation, or appressorium formation in Ph. infestans. Further analyses revealed that β-rubromycin inhibited th

SUBMITTER: Tani S 

PROVIDER: S-EPMC7749174 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature