Single-cell transcriptomic profiling provides insights into retinal endothelial barrier properties.

ABSTRACT:

Purpose

To better characterize retinal endothelial barrier properties through analysis of individual transcriptomes of primary bovine retinal microvascular endothelial cells (RMECs).

Methods

Individual RMECs were captured on the Fluidigm C1 system, cDNA libraries were prepared using a Nextera XT kit, and sequencing was performed on a NextSeq system (Illumina). Data analysis was performed using R packages Scater, SC3, and Seurat, and the browser application Automated Single-cell Analysis Pipeline (ASAP). Alternative splicing events in single cells were quantified with Outrigger. Cytoscape was used for network analyses.

Results

Application of a single-cell RNA sequencing (scRNA-seq) analysis workflow showed that RMECs form a relatively homogeneous population in culture,

SUBMITTER: Watson MI 

PROVIDER: S-EPMC7765706 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature