Dataset Information

Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana.

ABSTRACT: Globally, Staphylococcus aureus is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance of S. aureus to locally available antibiotics is increasing but the molecular basis of resistance and the population structure of S. aureus in particular in chronic wounds are poorly described. However, this information is essential to understand the underlying mechanisms of resistance and spread of resistant clones. We therefore subjected 28 S. aureus isolates from chronic infected wounds in a rural area of Ghana to whole genome sequencing. Overall, resistance of S. aureus to locally available antibiotics was high and 29% were Methicillin resistant Staphylococcus aureus (MRSA). The most abundant sequence type was ST88 (29%, 8/28) followed by ST152 (18%, 5/28). All ST88 carried the mecA gene, which was associated with this sequence type only. Chloramphenicol resistance gene fexB was exclusively associated with the methicillin-resistant ST88 strains. Panton-Valentine leukocidin (PVL) carriage was associated with ST121 and ST152. Other detected mechanisms of resistance included dfrG, conferring resistance to trimethoprim. This study provides valuable information for understanding the population structure and resistance mechanisms of S. aureus isolated from chronic wound infections in rural Ghana.

SUBMITTER: Wolters M

PROVIDER: S-EPMC7767444 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Molecular Characterization of <i>Staphylococcus aureus</i> Isolated from Chronic Infected Wounds in Rural Ghana.

Wolters Manuel M Frickmann Hagen H Christner Martin M Both Anna A Rohde Holger H Oppong Kwabena K Akenten Charity Wiafe CW May Jürgen J Dekker Denise D

Microorganisms 20201221 12

<h4>Background</h4>Globally, <i>Staphylococcus aureus</i> is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance of <i>S. aureus</i> to locally available antibiotics is increasing but the molecular basis of resistance and the population structure of <i>S. aureus</i> in particular in chronic wounds are poorly described. However, this information is essential to understand the underlying mechanisms of resistance and spread of res ...[more]

PMID: 33371449

Similar Datasets

Project description:Staphylococcus aureus is a commensal resident of the skin and nasal cavities of humans and can cause various infections. Some toxigenic strains can contaminate food matrices and cause foodborne intoxications. The present study aimed to provide relevant information (clonal complex lineages, agr types, virulence and antimicrobial resistance-associated genes) based on DNA microarray analyses as well as the origins and dissemination of several circulating clones of 60 Staphylococcus aureus isolated from food matrices (n = 24), clinical samples (n = 20), and nasal carriers (n = 16) in northern Algeria. Staphylococcus aureus were genotyped into 14 different clonal complexes. Out of 60 S. aureus, 13 and 10 isolates belonged to CC1-MSSA and CC97-MSSA, respectively. The CC 80-MRSA-IV was the predominant S. aureus strain in clinical isolates. The accessory gene regulator allele agr group III was mainly found among clinical isolates (70.4%). Panton-Valentine leukocidin genes lukF/lukS-PV were detected in 13.3% of isolates that all belonged to CC80-MRSA. The lukF/S-hlg, hlgA, and hla genes encoding for hemolysins and leucocidin components were detected in all Staphylococcusaureus isolates. Clinical and food isolates harbored more often the antibiotic resistance genes markers. Seventeen (28.3%) methicillin-resistant Staphylococcus aureus carrying the mecA gene localized on a SCCmec type IV element were identified. The penicillinase operon (blaZ/I/R) was found in 71.7% (43/60) of isolates. Food isolates belonging to CC97-MSSA carried several antibiotic resistance genes (blaZ, ermB, aphA3, sat, tetM, and tetK). The results of this study showed that all clones were found in their typical host, but interestingly, some nasal carriers had isolates assigned to CC705 thought to be absent in humans. The detection of MRSA strains among food isolates should be considered as a potential public health risk. Therefore, controlling the antibiotics prescription for a rational use in human and animal infections is mandatory.

Dataset Information

Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana.

Publications

Molecular Characterization of <i>Staphylococcus aureus</i> Isolated from Chronic Infected Wounds in Rural Ghana.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets