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Upregulation of a KN1 homolog by transposon insertion promotes leafy head development in lettuce.

ABSTRACT: Leafy head is a unique type of plant architecture found in some vegetable crops, with leaves bending inward to form a compact head. The genetic and molecular mechanisms underlying leafy head in vegetables remain poorly understood. We genetically fine-mapped and cloned a major quantitative trait locus controlling heading in lettuce. The candidate gene (LsKN1) is a homolog of knotted 1 (KN1) from Zea mays Complementation and CRISPR/Cas9 knockout experiments confirmed the role of LsKN1 in heading. In heading lettuce, there is a CACTA-like transposon inserted into the first exon of LsKN1 (LsKN1▽). The transposon sequences act as a promoter rather than an enhancer and drive high expression of LsKN1▽. The enhanced expression of LsKN1▽ is necessary but not sufficient for heading in lettuce. Data from ChIP-sequencing, electrophoretic mobility shift assays, and dual luciferase assays indicate that the LsKN1▽ protein binds the promoter of LsAS1 and down-regulates its expression to alter leaf dorsoventrality. This study provides insight into plant leaf development and will be useful for studies on heading in other vegetable crops.

SUBMITTER: Yu C

PROVIDER: S-EPMC7776633 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Upregulation of a KN1 homolog by transposon insertion promotes leafy head development in lettuce.

Yu Changchun C Yan Chenghuan C Liu Yuling Y Liu Yali Y Jia Yue Y Lavelle Dean D An Guanghui G Zhang Weiyi W Zhang Lei L Han Rongkui R Larkin Robert M RM Chen Jiongjiong J Michelmore Richard W RW Kuang Hanhui H

Proceedings of the National Academy of Sciences of the United States of America 20201207 52

Leafy head is a unique type of plant architecture found in some vegetable crops, with leaves bending inward to form a compact head. The genetic and molecular mechanisms underlying leafy head in vegetables remain poorly understood. We genetically fine-mapped and cloned a major quantitative trait locus controlling heading in lettuce. The candidate gene (LsKN1) is a homolog of knotted 1 (KN1) from Zea mays Complementation and CRISPR/Cas9 knockout experiments confirmed th ...[more]

PMID: 33288708

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Upregulation of a KN1 homolog by transposon insertion promotes leafy head development in lettuce.

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Upregulation of a <i>KN1</i> homolog by transposon insertion promotes leafy head development in lettuce.

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