Adaptive thermogenesis in brown adipose tissue involves activation of pannexin-1 channels.

ABSTRACT:

Objective

Brown adipose tissue (BAT) is specialized in thermogenesis. The conversion of energy into heat in brown adipocytes proceeds via stimulation of β-adrenergic receptor (βAR)-dependent signaling and activation of mitochondrial uncoupling protein 1 (UCP1). We have previously demonstrated a functional role for pannexin-1 (Panx1) channels in white adipose tissue; however, it is not known whether Panx1 channels play a role in the regulation of brown adipocyte function. Here, we tested the hypothesis that Panx1 channels are involved in brown adipocyte activation and thermogenesis.

Methods

In an immortalized brown pre-adipocytes cell line, Panx1 currents were measured using patch-clamp electrophysiology. Flow cytometry was used for assessment of dye uptake and luminescence a

SUBMITTER: Senthivinayagam S 

PROVIDER: S-EPMC7779784 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature