Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans.

ABSTRACT:

Background

Dyslipidaemia is a major risk factor for atherosclerosis and cardiovascular diseases. The molecular mechanisms that translate dyslipidaemia into atherogenesis and reliable markers of its progression are yet to be fully elucidated. To address this issue, we conducted a comprehensive metabolomic and proteomic analysis in an experimental model of dyslipidaemia and in patients with familial hypercholesterolemia (FH).

Methods

Liquid chromatography/mass spectrometry (LC/MS) and immunoassays were used to find out blood alterations at metabolite and protein levels in dyslipidaemic ApoE^-/-/LDLR^-/- mice and in FH patients to evaluate their human relevance.

Results

We identified 15 metabolites (inhibitors and substrates of nitric oxide synthase (

SUBMITTER: Olkowicz M 

PROVIDER: S-EPMC7789501 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature