The Role of MicroRNAs and Human Epidermal Growth Factor Receptor 2 in Proliferative Lupus Nephritis.

ABSTRACT:

Objective

To understand the roles of microRNAs (miRNAs) in proliferative lupus nephritis (LN).

Methods

A high-throughput analysis of the miRNA pattern of the kidneys of LN patients and controls was performed by molecular digital detection. Urinary miRNAs were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Target gene expression in human mesangial cells was evaluated by arrays and qRT-PCR. Human epidermal growth factor receptor 2 (HER-2) was analyzed by immunohistochemistry in kidney samples from LN patients and in a murine model of lupus. Urinary levels of HER-2, monocyte chemotactic protein 1 (MCP-1), and vascular cell adhesion molecule 1 (VCAM-1) were measured by enzyme-linked immunosorbent assay.

Results

Levels of the miRNAs miR

SUBMITTER: Costa-Reis P 

PROVIDER: S-EPMC7814461 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature