Dataset Information

Genome-wide identification of neuropeptides and their receptor genes in Bemisia tabaci and their transcript accumulation change in response to temperature stresses.

ABSTRACT: Insect neuropeptides play an important role in regulating physiological functions such as growth, development, behavior and reproduction. We identified temperature-sensitive neuropeptides and receptor genes of the cotton whitefly, Bemisia tabaci. We identified 38 neuropeptide precursor genes and 35 neuropeptide receptors and constructed a phylogenetic tree using additional data from other insects. As temperature adaptability enables B. tabaci to colonize a diversity of habitats, we performed quantitative polymerase chain reaction with two temperature stresses (low = 4 °C and high = 40 °C) to screen for temperature-sensitive neuropeptides. We found many neuropeptides and receptors that may be involved in the temperature adaptability of B. tabaci. This study is the first to identify B. tabaci neuropeptides and their receptors, and it will help to reveal the roles of neuropeptides in temperature adaptation of B. tabaci.

SUBMITTER: Li JJ

PROVIDER: S-EPMC7818427 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Genome-wide identification of neuropeptides and their receptor genes in Bemisia tabaci and their transcript accumulation change in response to temperature stresses.

Li Jiang-Jie JJ Shi Yan Y Lin Gan-Lin GL Yang Chun-Hong CH Liu Tong-Xian TX

Insect science 20200525 1

Insect neuropeptides play an important role in regulating physiological functions such as growth, development, behavior and reproduction. We identified temperature-sensitive neuropeptides and receptor genes of the cotton whitefly, Bemisia tabaci. We identified 38 neuropeptide precursor genes and 35 neuropeptide receptors and constructed a phylogenetic tree using additional data from other insects. As temperature adaptability enables B. tabaci to colonize a diversity of habitats, we performed qua ...[more]

PMID: 31912953

Similar Datasets

Project description:The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), and the viruses it transmits, are a major constraint to vegetable crops, worldwide. Although the whitefly is usually controlled using chemical pesticides, biological control agents constitute an important component in integrated pest management programs. One of these agents is the wasp Eretmocerus mundus (Mercet) (Hymenoptera: Aphelinidae). E. mundus lays its egg on the leaf underneath the pupa of B. tabaci. First instars of the wasp hatch and penetrate the whitefly larvae. Initiation of parasitization induces the host to form a cellular capsule around the parasitoid. Around this capsule, epidermal cells multiply and thick layers of cuticle are deposited. The physiological and molecular processes underlying B. tabaci-E. mundus interactions have not been investigated so far. We have used a cDNA microarray containing 6,000 expressed sequence tags (ESTs) from the whitefly genome to study the parasitoid-whitefly interaction. We compared RNA samples collected across two time points of the parasitization process: when the parasitoid first instar started the penetration process and once it had fully penetrated the host. The results clearly indicated that genes known to be part of the defense pathways described in other insects are also involved in the response of B. tabaci to parasitization by E. mundus. Some of the responses observed included the repression of a serine protease inhibitor (serpin) and the induction of a melanization cascade. A second set of genes that strongly responded to parasitization included bacterial genes encoded by whitefly symbionts. Quantitative real-time PCR and FISH analyses showed that proliferation of Rickettsia, a facultative secondary symbiont, was strongly induced following the initiation of the parasitization process, a result that supported previous reports suggesting that endosymbionts may be involved in the insect host resistance to various environmental stresses. This is the first study examining the transcriptional response of a hemipteran insect to the attack of a biological control agent (Hymenopterous parasitoid), using a new genomic approach developed for this insect pest. The defense response in B. tabaci seems to resemble that of model organisms such as Drosophila melanogaster. Moreover, endosymbionts of B. tabaci seem to play a role in the response to parasitization, and this is supported by previously published results from aphids. Keywords: time course

Dataset Information

Genome-wide identification of neuropeptides and their receptor genes in Bemisia tabaci and their transcript accumulation change in response to temperature stresses.

Publications

Genome-wide identification of neuropeptides and their receptor genes in Bemisia tabaci and their transcript accumulation change in response to temperature stresses.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets