TRNA-derived fragments tRF^GlnCTG induced by arterial injury promote vascular smooth muscle cell proliferation.

ABSTRACT: tRNA-derived fragments (tRFs) and tRNA halves (tiRNAs) are originated from the specific cleavage of endogenous tRNAs or their precursors and regulate gene expression when the cells are in stressful circumstances. Here, we replicated the rat common carotid artery (CCA) intimal hyperplasia model and investigated the expression of tRFs/tiRNAs in the artery. The normal and the balloon-injured rat CCAs were subjected to small RNA sequencing, and then the differentially expressed tRFs/tiRNAs were identified and analyzed. The expression profiles of tRFs/tiRNAs in the healthy and injured CCAs were remarkably different. tRNA^GlnCTG-derived fragments (tRF^GlnCTG) were found to be overexpressed with a high abundance in the injured CCA. In in vitro experiments, the synthetic

SUBMITTER: Zhu XL 

PROVIDER: S-EPMC7819823 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature