Real-Time Culture-Independent Microbial Profiling Onboard the International Space Station Using Nanopore Sequencing.

ABSTRACT: For the past two decades, microbial monitoring of the International Space Station (ISS) has relied on culture-dependent methods that require return to Earth for analysis. This has a number of limitations, with the most significant being bias towards the detection of culturable organisms and the inherent delay between sample collection and ground-based analysis. In recent years, portable and easy-to-use molecular-based tools, such as Oxford Nanopore Technologies' MinION™ sequencer and miniPCR bio's miniPCR™ thermal cycler, have been validated onboard the ISS. Here, we report on the development, validation, and implementation of a swab-to-sequencer method that provides a culture-independent solution to real-time microbial profiling onboard the ISS. Method development focused on analysis of s

SUBMITTER: Stahl-Rommel S 

PROVIDER: S-EPMC7830261 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature