Metabolomic and Lipidomic Analysis of Bone Marrow Derived Macrophages.

ABSTRACT: Macrophages are highly plastic immune cells that are capable of adopting a wide array of functional phenotypes in response to environmental stimuli. The changes in macrophage function are often supported and regulated by changes in cellular metabolism. Capturing a comprehensive picture of metabolism is vital for understanding the role of metabolic rewiring in the immune response. Here we present a method for systematically quantifying the abundance of metabolites and lipids in primary murine bone marrow derived macrophages (BMDMs). This method simultaneously extracts polar metabolites and lipids from BMDMs using a rapid two-phase extraction procedure. The polar metabolite fraction and lipid fraction are subsequently analyzed by separate liquid chromatography-mass spectrometry (LC-MS) metho

SUBMITTER: Seim GL 

PROVIDER: S-EPMC7842587 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature