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Genomic monitoring of SARS-CoV-2 uncovers an Nsp1 deletion variant that modulates type I interferon response.

ABSTRACT: The SARS-CoV-2 virus, the causative agent of COVID-19, is undergoing constant mutation. Here, we utilized an integrative approach combining epidemiology, virus genome sequencing, clinical phenotyping, and experimental validation to locate mutations of clinical importance. We identified 35 recurrent variants, some of which are associated with clinical phenotypes related to severity. One variant, containing a deletion in the Nsp1-coding region (Δ500-532), was found in more than 20% of our sequenced samples and associates with higher RT-PCR cycle thresholds and lower serum IFN-β levels of infected patients. Deletion variants in this locus were found in 37 countries worldwide, and viruses isolated from clinical samples or engineered by reverse genetics with related deletions in Nsp1 also induce lower IFN-β responses in infected Calu-3 cells. Taken together, our virologic surveillance characterizes recurrent genetic diversity and identified mutations in Nsp1 of biological and clinical importance, which collectively may aid molecular diagnostics and drug design.

SUBMITTER: Lin JW

PROVIDER: S-EPMC7846228 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Genomic monitoring of SARS-CoV-2 uncovers an Nsp1 deletion variant that modulates type I interferon response.

Lin Jing-Wen JW Tang Chao C Wei Han-Cheng HC Du Baowen B Chen Chuan C Wang Minjin M Zhou Yongzhao Y Yu Ming-Xia MX Cheng Lu L Kuivanen Suvi S Ogando Natacha S NS Levanov Lev L Zhao Yuancun Y Li Chang-Ling CL Zhou Ran R Li Zhidan Z Zhang Yiming Y Sun Ke K Wang Chengdi C Chen Li L Xiao Xia X Zheng Xiuran X Chen Sha-Sha SS Zhou Zhen Z Yang Ruirui R Zhang Dan D Xu Mengying M Song Junwei J Wang Danrui D Li Yupeng Y Lei ShiKun S Zeng Wanqin W Yang Qingxin Q He Ping P Zhang Yaoyao Y Zhou Lifang L Cao Ling L Luo Feng F Liu Huayi H Wang Liping L Ye Fei F Zhang Ming M Li Mengjiao M Fan Wei W Li Xinqiong X Li Kaiju K Ke Bowen B Xu Jiannan J Yang Huiping H He Shusen S Pan Ming M Yan Yichen Y Zha Yi Y Jiang Lingyu L Yu Changxiu C Liu Yingfen Y Xu Zhiyong Z Li Qingfeng Q Jiang Yongmei Y Sun Jiufeng J Hong Wei W Wei Hongping H Lu Guangwen G Vapalahti Olli O Luo Yunzi Y Wei Yuquan Y Connor Thomas T Tan Wenjie W Snijder Eric J EJ Smura Teemu T Li Weimin W Geng Jia J Ying Binwu B Ying Binwu B Chen Lu L

Cell host & microbe 20210129 3

The SARS-CoV-2 virus, the causative agent of COVID-19, is undergoing constant mutation. Here, we utilized an integrative approach combining epidemiology, virus genome sequencing, clinical phenotyping, and experimental validation to locate mutations of clinical importance. We identified 35 recurrent variants, some of which are associated with clinical phenotypes related to severity. One variant, containing a deletion in the Nsp1-coding region (Δ500-532), was found in more than 20% of our sequence ...[more]

PMID: 33548198

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Project description:BackgroundThe new Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), which was first detected in Wuhan (China) in December of 2019 is responsible for the current global pandemic. Phylogenetic analysis revealed that it is similar to other betacoronaviruses, such as SARS-CoV and Middle-Eastern Respiratory Syndrome, MERS-CoV. Its genome is ∼ 30 kb in length and contains two large overlapping polyproteins, ORF1a and ORF1ab that encode for several structural and non-structural proteins. The non-structural protein 1 (nsp1) is arguably the most important pathogenic determinant, and previous studies on SARS-CoV indicate that it is both involved in viral replication and hampering the innate immune system response. Detailed experiments of site-specific mutagenesis and in vitro reconstitution studies determined that the mechanisms of action are mediated by (a) the presence of specific amino acid residues of nsp1 and (b) the interaction between the protein and the host's small ribosomal unit. In fact, substitution of certain amino acids resulted in reduction of its negative effects.MethodsA total of 17,928 genome sequences were obtained from the GISAID database (December 2019 to July 2020) from patients infected by SARS-CoV-2 from different areas around the world. Genomes alignment was performed using MAFFT (REFF) and the nsp1 genomic regions were identified using BioEdit and verified using BLAST. Nsp1 protein of SARS-CoV-2 with and without deletion have been subsequently modelled using I-TASSER.ResultsWe identified SARS-CoV-2 genome sequences, from several Countries, carrying a previously unknown deletion of 9 nucleotides in position 686-694, corresponding to the AA position 241-243 (KSF). This deletion was found in different geographical areas. Structural prediction modelling suggests an effect on the C-terminal tail structure.ConclusionsModelling analysis of a newly identified deletion of 3 amino acids (KSF) of SARS-CoV-2 nsp1 suggests that this deletion could affect the structure of the C-terminal region of the protein, important for regulation of viral replication and negative effect on host's gene expression. In addition, substitution of the two amino acids (KS) from nsp1 of SARS-CoV was previously reported to revert loss of interferon-alpha expression. The deletion that we describe indicates that SARS-CoV-2 is undergoing profound genomic changes. It is important to: (i) confirm the spreading of this particular viral strain, and potentially of strains with other deletions in the nsp1 protein, both in the population of asymptomatic and pauci-symptomatic subjects, and (ii) correlate these changes in nsp1 with potential decreased viral pathogenicity.

Dataset Information

Genomic monitoring of SARS-CoV-2 uncovers an Nsp1 deletion variant that modulates type I interferon response.

Publications

Genomic monitoring of SARS-CoV-2 uncovers an Nsp1 deletion variant that modulates type I interferon response.

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