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Potentilla anserina L. developmental changes affect the rhizosphere prokaryotic community.

ABSTRACT: Plant roots and soil prokaryotes primarily interact with each other in the rhizosphere. Changes in the rhizosphere prokaryotic structure are influenced by several factors. In this study, the community structure of the Potentilla anserina L. rhizosphere prokaryotes was identified and evaluated by high-throughput sequencing technology in different continuous cropping fields and developmental stages of the plant. In total, 2 archaeal (Euryarchaeota and Thaumarchaeota) and 26 bacterial phyla were identified in the P. anserina rhizosphere. The bacterial community was mainly composed of Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Gemmatimonadetes, Planctomycetes, Proteobacteria, and Verrucomicrobia. Moreover, the prokaryotic community structure of the rhizosphere varied significantly during plant development. Our results provide new insights into the dynamics of the P. anserina rhizosphere prokaryotic community and may provide useful information for enhancing the growth and development of P. anserina through artificial control of the soil prokaryotes.

SUBMITTER: Wang Y

PROVIDER: S-EPMC7854623 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Potentilla anserina L. developmental changes affect the rhizosphere prokaryotic community.

Wang Yaqiong Y Liu Yuxi Y Li Xue X Han Xiaoyan X Zhang Zhen Z Ma Xiaoling X Li Junqiao J

Scientific reports 20210202 1

Plant roots and soil prokaryotes primarily interact with each other in the rhizosphere. Changes in the rhizosphere prokaryotic structure are influenced by several factors. In this study, the community structure of the Potentilla anserina L. rhizosphere prokaryotes was identified and evaluated by high-throughput sequencing technology in different continuous cropping fields and developmental stages of the plant. In total, 2 archaeal (Euryarchaeota and Thaumarchaeota) and 26 bacterial phyla were id ...[more]

PMID: 33531629

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Project description:BackgroundRhizosphere microbial communities are key regulators of plant performance, yet few studies have assessed the impact of different management approaches on the rhizosphere microbiomes of major crops. Rhizosphere microbial communities are shaped by interactions between agricultural management and host selection processes, but studies often consider these factors individually rather than in combination. We tested the impacts of management (M) and rhizosphere effects (R) on microbial community structure and co-occurrence networks of maize roots collected from long-term conventionally and organically managed maize-tomato agroecosystems. We also explored the interaction between these factors (M × R) and how it impacts rhizosphere microbial diversity and composition, differential abundance, indicator taxa, co-occurrence network structure, and microbial nitrogen-cycling processes.ResultsHost selection processes moderate the influence of agricultural management on rhizosphere microbial communities, although bacteria and fungi respond differently to plant selection and agricultural management. We found that plants recruit management-system-specific taxa and shift N-cycling pathways in the rhizosphere, distinguishing this soil compartment from bulk soil. Rhizosphere microbiomes from conventional and organic systems were more similar in diversity and network structure than communities from their respective bulk soils, and community composition was affected by both M and R effects. In contrast, fungal community composition was affected only by management, and network structure only by plant selection. Quantification of six nitrogen-cycling genes (nifH, amoA [bacterial and archaeal], nirK, nrfA, and nosZ) revealed that only nosZ abundance was affected by management and was higher in the organic system.ConclusionsPlant selection interacts with conventional and organic management practices to shape rhizosphere microbial community composition, co-occurrence patterns, and at least one nitrogen-cycling process. Reframing research priorities to better understand adaptive plant-microbe feedbacks and include roots as a significant moderating influence of management outcomes could help guide plant-oriented strategies to improve productivity and agroecosystem sustainability.

Project description:BackgroundThe tuberous roots of Potentilla anserina (Pan), which are called silverweed cinquefoil roots, serve as a source of starch for the inhabitants of the Qinghai-Tibet Plateau. They are also regarded as a valuable tonic food and herbal medicine in the ethnic medicine system. Starch plays a crucial part in the plant's life cycle, particularly during the growth stage and in response to abiotic stress. Moreover, numerous biological processes and regulatory networks are involved in the synthesis and accumulation of starch.ResultsIn this research, a combination of transcriptome and metabolomics approaches were employed to analyze the genes related to starch synthesis and degradation in Pan. The crucial genes involved in starch metabolism were identified, and the response characteristics of these genes to drought and low temperature treatments were investigated. Seven AMYs (Alpha-amylases) and 18 BAMs (Beta-amylases) were identified from the genome of Pan. Molecular phylogenetic analyses of AMYs and BAMs derived from 11 species of rosids were conducted respectively. AMYs of Pan and other species were clustered into 3 groups, whereas BAMs were classified into 4 groups. In the tuberous roots, when compared with the control conditions, 2 AMYs and 4 BAMs were upregulated, while 3 BAMs were downregulated, meanwhile, the contents of maltose and glucose-6-phosphate (G6P) were decreased, while the content of glucose-1-phosphate (G1P) was increased under drought treatment. In the tuberous roots under low temperature treatment, 1 BAM was upregulated, while 2 AMYs and 4 BAMs were downregulated compared with control. The response characteristics of AMYs and BAMs to drought and low temperature treatments were further verified through qPCR analysis.ConclusionsIn this research, the genes associated with starch synthesis in Pan were characterized, and the functions of AMYs and BAMs in abiotic stress treatments were elucidated. An overview of evolution of AMY and BAM gene families within rosids was also provided.

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Potentilla anserina L. developmental changes affect the rhizosphere prokaryotic community.

Publications

Potentilla anserina L. developmental changes affect the rhizosphere prokaryotic community.

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