Transient light-activated gene expression in Chinese hamster ovary cells.

ABSTRACT:

Background

Chinese hamster ovary (CHO) cells are widely used for industrial production of biopharmaceuticals. Many genetic, chemical, and environmental approaches have been developed to modulate cellular pathways to improve titers. However, these methods are often irreversible or have off-target effects. Development of techniques which are precise, tunable, and reversible will facilitate temporal regulation of target pathways to maximize titers. In this study, we investigate the use of optogenetics in CHO cells. The light-activated CRISPR-dCas9 effector (LACE) system was first transiently transfected to express eGFP in a light-inducible manner. Then, a stable system was tested using lentiviral transduction.

Results

Transient transfections resulted in increasing eGFP expressi

SUBMITTER: Minami SA 

PROVIDER: S-EPMC7863527 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature