Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton.

ABSTRACT:

Background

Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functional analyses extremely challenging. The recent accessibility of CRISPR/Cas9 tool provides the ability to modify targeted locus efficiently in various complicated plant genomes. However, current cotton transformation method targeting one gene requires a complicated, long and laborious regeneration process. Hence, optimizing strategy that targeting multiple genes is of great value in cotton functional genomics and genetic engineering.

Results

To target multiple genes in a single experiment, 112 plant development-related genes were knocked out via optimized C

SUBMITTER: Ramadan M 

PROVIDER: S-EPMC7869495 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature