An Integrated Approach Based on Network Pharmacology Combined with Experimental Verification Reveals AMPK/PI3K/Akt Signaling is an Important Way for the Anti-Type 2 Diabetic Activity of Silkworm Excrement.

ABSTRACT:

Objective

This study was aimed to investigate the potential active components, targets and mechanisms of silkworm excrement (SE) in the treatment of type 2 diabetes mellitus (T₂D) based on THE network pharmacology combined with experimental verification.

Methods

Firstly, the inhibitory effects of SE on α-glucosidase were measured in vitro. Then, the potential active components and potential targets of SE and the targets of T₂D were collected and screened using bioinformatics databases. Then, the R language, Cytoscape, Perl software were used to screen and visualize important components, targets, biological processes and signaling pathways. Finally, the predicted results by network pharmacology were verified via glucose absorption assay, oil red O staini

SUBMITTER: Duan H 

PROVIDER: S-EPMC7887153 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature