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Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells.

ABSTRACT: Lactoferrin is part of the innate immune system, with antiviral activity against numerous DNA and RNA viruses. Rhinoviruses, the leading cause of the common cold, are associated with exacerbation of respiratory illnesses such as asthma. Here, we explored the effect of bovine lactoferrin (BLf) on RV-B14 infectivity. Using different assays, we show that the effect of BLf is strongest during adhesion of the virus to the cell and entry. Tracking the internalisation of BLf and virus revealed a degree of colocalisation, although their interaction was only confirmed in vitro using empty viral particles, indicating a possible additional influence of BLf on other infection steps.

SUBMITTER: Denani CB

PROVIDER: S-EPMC7894240 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells.

Denani Caio Bidueira CB Real-Hohn Antonio A de Carvalho Carlos Alberto Marques CAM Gomes Andre Marco de Oliveira AMO Gonçalves Rafael Braga RB

Archives of virology 20210219 4

Lactoferrin is part of the innate immune system, with antiviral activity against numerous DNA and RNA viruses. Rhinoviruses, the leading cause of the common cold, are associated with exacerbation of respiratory illnesses such as asthma. Here, we explored the effect of bovine lactoferrin (BLf) on RV-B14 infectivity. Using different assays, we show that the effect of BLf is strongest during adhesion of the virus to the cell and entry. Tracking the internalisation of BLf and virus revealed a degree ...[more]

PMID: 33606112

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