Cytotrophoblasts suppress macrophage-mediated inflammation through a contact-dependent mechanism.

ABSTRACT:

Problem

Gestational membrane (GM) infection provokes inflammation and can result in preterm prelabor rupture of membranes (PPROM). The choriodecidual layer of the GM includes decidual stromal cells (DSC), cytotrophoblasts (CTB), and macrophages (Mφ). Our laboratory has previously shown that DSCs suppress Mφ TNF-α production through secreted prostaglandin E₂ . We hypothesized that CTBs would also inhibit Mφ cytokine expression through secreted mediators.

Method of study

THP.1 Mφ-like cells with an NF-κB reporter construct or human blood monocyte-derived Mφ were co-cultured with the Jeg3 CTB cell line or primary human CTBs and challenged with group B streptococcus (GBS) or Toll-like receptor (TLR) agonists. Conditioned medium generated from CTB cultures was applied

SUBMITTER: Eastman AJ 

PROVIDER: S-EPMC7897285 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature