Deepening Our Roots and Growing Wings.
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ABSTRACT:
SUBMITTER: Zhang D
PROVIDER: S-EPMC7905803 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
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Project description:Control over cell growth by mobile regulators underlies much of eukaryotic morphogenesis. In plant roots, cell division and elongation are separated into distinct longitudinal zones and both division and elongation are influenced by the growth regulatory hormone gibberellin (GA). Previously, a multicellular mathematical model predicted a GA maximum at the border of the meristematic and elongation zones. However, GA in roots was recently measured using a genetically encoded fluorescent biosensor, nlsGPS1, and found to be low in the meristematic zone grading to a maximum at the end of the elongation zone. Furthermore, the accumulation rate of exogenous GA was also found to be higher in the elongation zone. It was still unknown which biochemical activities were responsible for these mobile small molecule gradients and whether the spatiotemporal correlation between GA levels and cell length is important for root cell division and elongation patterns. Using a mathematical modeling approach in combination with high-resolution GA measurements in vivo, we now show how differentials in several biosynthetic enzyme steps contribute to the endogenous GA gradient and how differential cellular permeability contributes to an accumulation gradient of exogenous GA. We also analyzed the effects of altered GA distribution in roots and did not find significant phenotypes resulting from increased GA levels or signaling. We did find a substantial temporal delay between complementation of GA distribution and cell division and elongation phenotypes in a GA deficient mutant. Together, our results provide models of how GA gradients are directed and in turn direct root growth.
| S-EPMC7923382 | biostudies-literature
Project description:To understand dynamic developmental processes, living tissues have to be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring continuous specimen access, while preserving physiological conditions and preventing photo-damage, poses major barriers to measurements of cellular dynamics in growing organs such as plant roots. We present a system that integrates optical sectioning through light sheet fluorescence microscopy with hydroponic culture that enables us to image, at cellular resolution, a vertically growing Arabidopsis root every few minutes and for several consecutive days. We describe novel automated routines to track the root tip as it grows, to track cellular nuclei and to identify cell divisions. We demonstrate the system's capabilities by collecting data on divisions and nuclear dynamics.
| S-EPMC3120859 | biostudies-literature
Project description:We present a software platform for reconstructing and analyzing the growth of a plant root system from a time-series of 3D voxelized shapes. It aligns the shapes with each other, constructs a geometric graph representation together with the function that records the time of growth, and organizes the branches into a hierarchy that reflects the order of creation. The software includes the automatic computation of structural and dynamic traits for each root in the system enabling the quantification of growth on fine-scale. These are important advances in plant phenotyping with applications to the study of genetic and environmental influences on growth.
| S-EPMC4452513 | biostudies-literature
Project description:We report observations of acoustic emissions (AE) from growing plant roots and burrowing earthworms in soil, as a noninvasive method for monitoring biophysical processes that modify soil structure. AE emanating from earthworm and plants root activity were linked with time-lapse imaging in glass cells. Acoustic waveguides where installed in soil columns to monitor root growth in real time (mimicking field application). The cumulative AE events were in correlation with earthworm burrow lengths and with root growth. The number of AE events recorded from the soil columns with growing maize roots were several orders of magnitude larger than AE emanating from bare soil under similar conditions. The results suggest that AE monitoring may offer a window into largely unobservable dynamics of soil biomechanical processes such as root growth or patterns of earthworm activity - both important soil structure forming processes.
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Project description:Study objectivesSlow wave sleep (SWS) plays a critical role in body restoration and promotes brain plasticity; however, it markedly declines across the lifespan. Despite its importance, effective tools to increase SWS are rare. Here we tested whether a hypnotic suggestion to "sleep deeper" extends the amount of SWS.DesignWithin-subject, placebo-controlled crossover design.SettingSleep laboratory at the University of Zurich, Switzerland.ParticipantsSeventy healthy females 23.27 ± 3.17 y.InterventionParticipants listened to an auditory text with hypnotic suggestions or a control tape before napping for 90 min while high-density electroencephalography was recorded.Measurements and resultsAfter participants listened to the hypnotic suggestion to "sleep deeper" subsequent SWS was increased by 81% and time spent awake was reduced by 67% (with the amount of SWS or wake in the control condition set to 100%). Other sleep stages remained unaffected. Additionally, slow wave activity was significantly enhanced after hypnotic suggestions. During the hypnotic tape, parietal theta power increases predicted the hypnosis-induced extension of SWS. Additional experiments confirmed that the beneficial effect of hypnotic suggestions on SWS was specific to the hypnotic suggestion and did not occur in low suggestible participants.ConclusionsOur results demonstrate the effectiveness of hypnotic suggestions to specifically increase the amount and duration of slow wave sleep (SWS) in a midday nap using objective measures of sleep in young, healthy, suggestible females. Hypnotic suggestions might be a successful tool with a lower risk of adverse side effects than pharmacological treatments to extend SWS also in clinical and elderly populations.
| S-EPMC4015388 | biostudies-literature
Project description:Introduction: High-density lipoprotein (HDL) particles are heterogeneous and their proteome is complex and distinct from HDL cholesterol. However, it is largely unknown whether HDL proteins are associated with cardiovascular protection. Areas covered: HDL isolation techniques and proteomic analyses are reviewed. A list of HDL proteins reported in 37 different studies was compiled and the effects of different isolation techniques on proteins attributed to HDL are discussed. Mass spectrometric techniques used for HDL analysis and the need for precise and robust methods for quantification of HDL proteins are discussed. Expert opinion: Proteins associated with HDL have the potential to be used as biomarkers and/or help to understand HDL functionality. To achieve this, large cohorts must be studied using precise quantification methods. Key factors in HDL proteome quantification are the isolation methodology and the mass spectrometry technique employed. Isolation methodology affects what proteins are identified in HDL and the specificity of association with HDL particles needs to be addressed. Shotgun proteomics yields imprecise quantification, but the majority of HDL studies relied on this approach. Few recent studies used targeted tandem mass spectrometry to quantify HDL proteins, and it is imperative that future studies focus on the application of these precise techniques.
| S-EPMC6802954 | biostudies-literature
Project description:Water is crucial for meeting sustainability targets, but its unsustainable use threatens human wellbeing and the environment. Past assessments of water scarcity (i.e., water demand in exceedance of availability) have often been spatially coarse and temporally limited, reducing their utility for targeting interventions. Here we perform a detailed monthly sub-basin assessment of the evolution of blue (i.e., surface and ground) water scarcity (years 1980-2015) for the world's three most populous countries - China, India, and the USA. Disaggregating by specific crops and sectors, we find that blue water demand rose by 60% (China), 71% (India), and 27% (USA), dominated by irrigation for a few key crops (alfalfa, maize, rice, wheat). We also find that unsustainable demand during peak months of use has increased by 101% (China), 82% (India), and 49% (USA) and that 32% (China), 61% (India), and 27% (US) of sub-basins experience at least 4 months of scarcity. These findings demonstrate that rising water demands are disproportionately being met by water resources in already stressed regions and provide a basis for targeting potential solutions that better balance the water needs of humanity and nature.
| S-EPMC11775186 | biostudies-literature
Project description:In this article, we present a novel class of robots that are able to move by growing and building their own structure. In particular, taking inspiration by the growing abilities of plant roots, we designed and developed a plant root-like robot that creates its body through an additive manufacturing process. Each robotic root includes a tubular body, a growing head, and a sensorized tip that commands the robot behaviors. The growing head is a customized three-dimensional (3D) printer-like system that builds the tubular body of the root in the format of circular layers by fusing and depositing a thermoplastic material (i.e., polylactic acid [PLA] filament) at the tip level, thus obtaining movement by growing. A differential deposition of the material can create an asymmetry that results in curvature of the built structure, providing the possibility of root bending to follow or escape from a stimulus or to reach a desired point in space. Taking advantage of these characteristics, the robotic roots are able to move inside a medium by growing their body. In this article, we describe the design of the growing robot together with the modeling of the deposition process and the description of the implemented growing movement strategy. Experiments were performed in air and in an artificial medium to verify the functionalities and to evaluate the robot performance. The results showed that the robotic root, with a diameter of 50 mm, grows with a speed of up to 4 mm/min, overcoming medium pressure of up to 37 kPa (i.e., it is able to lift up to 6 kg) and bending with a minimum radius of 100 mm.
| S-EPMC5649421 | biostudies-literature
Project description:Spatial and temporal profiling of metabolites within and between living systems is vital to understanding how chemical signaling shapes the composition and function of these complex systems. Measurement of metabolites is challenging because they are often not amenable to extrinsic tags, are diverse in nature, and are present with a broad range of concentrations. Moreover, direct imaging by chemically informative tools can significantly compromise viability of the system of interest or lack adequate resolution. Here, we present a nano-enabled and label-free imaging technology using a microfluidic sampling network to track production and distribution of chemical information in the microenvironment of a living organism. We describe the integration of a polyester track-etched (PETE) nanofluidic interface to physically confine the biological sample within the model environment, while allowing fluidic access via an underlying microfluidic network. The nanoporous interface enables sampling of the microenvironment above in a time-dependent and spatially-resolved manner. For demonstration, the diffusional flux through the PETE membrane was characterized to understand membrane performance, and exometabolites from a growing plant root were successfully profiled in a space- and time-resolved manner. This method and device provide a frame-by-frame description of the chemical environment that maps to the physical and biological characteristics of the sample.
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Project description:Several studies have demonstrated the relevance of endophytic bacteria on the growth and fitness of agriculturally-relevant plants. To our knowledge, however, little information is available on the composition, diversity, and interaction of endophytic bacterial communities in plants struggling for existence in the extreme environments of Chile, such as the Atacama Desert (AD) and Patagonia (PAT). The main objective of the present study was to analyze and compare the composition of endophytic bacterial communities associated with roots and leaves of representative plants growing in Chilean extreme environments. The plants sampled were: Distichlis spicate and Pluchea absinthioides from the AD, and Gaultheria mucronata and Hieracium pilosella from PAT. The abundance and composition of their endophytic bacterial communities was determined by quantitative PCR and high-throughput sequencing of 16S rRNA, respectively. Results indicated that there was a greater abundance of 16S rRNA genes in plants from PAT (1013 to 1014 copies g-1 DNA), compared with those from AD (1010 to 1012 copies g-1 DNA). In the AD, a greater bacterial diversity, as estimated by Shannon index, was found in P. absinthioides, compared with D. spicata. In both ecosystems, the greater relative abundances of endophytes were mainly attributed to members of the phyla Proteobacteria (14% to 68%), Firmicutes (26% to 41%), Actinobacteria (6 to 23%) and Bacteroidetes (1% to 21%). Our observations revealed that most of operational taxonomic units (OTUs) were not shared between tissue samples of different plant species in both locations, suggesting the effect of the plant genotype (species) on the bacterial endophyte communities in Chilean extreme environments, where Bacillaceae and Enterobacteriacea could serve as keystone taxa as revealed our linear discriminant analysis.
| S-EPMC6426880 | biostudies-literature