Exogenous NAD⁺ Postpones the D-Gal-Induced Senescence of Bone Marrow-Derived Mesenchymal Stem Cells via Sirt1 Signaling.

ABSTRACT: Cell senescence is accompanied by decreased nicotinamide adenine dinucleotide (NAD⁺) levels; however, whether exogenous NAD⁺ affects bone marrow-derived mesenchymal stem cells (BMSCs) senescence and the involved mechanisms is still unclear. Here, we find that exogenous NAD⁺ replenishment significantly postpones BMSC senescence induced by D-galactose (D-gal). It is also shown that exogenous NAD⁺ leads to increased intracellular NAD⁺ levels and reduced intracellular reactive oxygen species in senescent BMSCs here. Further investigation showed that exogenous NAD⁺ weakened BMSC senescence by increasing Sirtuin 1 (Sirt1) expression. Moreover, exogenous NAD⁺ reduced senescence-associated-?-galactosidase activity, and downregulated poly (ADP-ribose) polymerase 1 expression. In addition, the reduced expression of Sirt1 by small interfering RNA abolished the beneficial effects of exogenous NAD⁺ in terms of postponing BMSCs senescence induced by D-gal. Taken together, our results indicate that exogenous NAD⁺ could postpone D-gal-induced BMSC senescence through Sirt1 signaling, providing a potential method for obtaining high quality BMSCs to support their research and clinical application.

SUBMITTER: Wang J 

PROVIDER: S-EPMC7915830 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature