Molecular detection of Theileria annulata infection in cattle by conventional PCR and quantitative real time PCR in India.

ABSTRACT: Theileria annulata (T. annulata) is a tick-borne apicomplexan parasite that affects bovine. It is endemic in many tropical and subtropics areas, including Odisha, India. The objective of this study is to identify T. annulata infection in the peripheral blood of cattle as a biological sample by conventional PCR (cPCR) and quantitative PCR (qPCR). The phylogenetic analysis was done using the T. annulata merozoite surface antigen (Tams 1) gene. Out of 552 samples of examined blood smears by microscopy, 454 (82.24%) animals were positive for Theileria species. Out of 454 samples, 96 samples were further examined by both cPCR and qPCR, 52 samples (54.16%) were found positive for T. annulata in both PCR methodologies. Phylogenetic analysis reveale

SUBMITTER: Selim AM 

PROVIDER: S-EPMC7921255 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature