Versorgungsprojekt NPPV auf der Zielgeraden
Ontology highlight
ABSTRACT:
SUBMITTER: Nelles G
PROVIDER: S-EPMC7931789 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
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| S-EPMC8489974 | biostudies-literature
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| S-EPMC10947009 | biostudies-literature
Project description:Hintergrund Seit Beginn der COVID-19-Pandemie mehren sich Befunde zu ihrem negativen Einfluss auf die psychische Gesundheit von Kindern und Jugendlichen. Bisher ist jedoch wenig darüber bekannt, ob und wie sich dies auf die psychotherapeutische Versorgung von Kindern und Jugendlichen niederschlägt. Ziel der Arbeit Die psychische Situation von Kindern und Jugendlichen sowie ihre psychotherapeutische Versorgung seit Beginn der COVID-19-Pandemie sollen aus Sicht von Kinder- und JugendlichenpsychotherapeutInnen (KJP) erfasst werden. Material und Methoden Es wurden 324 KJP aus Deutschland in einer Online-Umfrage gebeten, die letzten 6 Monate mit einem 6‑monatigen Zeitraum vor 2 Jahren zu vergleichen. Fünf- und 7‑stufige Likert-Skalen, Fragen mit Mehr- und Einfachauswahl sowie numerische und ein freies Antwortformat wurden verwendet. Ergebnisse Seit Pandemiebeginn haben sich die Wartezeiten nahezu verdoppelt. Es werden mehr Behandlungsstunden angeboten, v. a. mehr Erstgespräche durchgeführt. Therapieverlängerungen kommen häufiger, -abbrüche seltener vor. Bei der Hälfte der PatientInnen ist eine pandemieassoziierte Symptomverschlechterung aufgetreten. Alle erfragten psychischen Störungen treten z. T. deutlich häufiger auf (v. a. Depressionen, Angststörungen, Medienabhängigkeit, Schlaf‑, Anpassungs‑, Zwangs- und Essstörungen). Es erfolgen mehr Telefon- und Videositzungen als vor der Pandemie. Die Zusammenarbeit mit Eltern hat sich verstärkt, die mit dem interdisziplinären Netzwerk verringert. Diskussion Die Pandemie hat einen deutlichen Einfluss auf die psychische Verfassung und die psychotherapeutische Versorgung von Kindern und Jugendlichen in Deutschland. Eine Anpassung des Versorgungssystems an den gestiegenen Bedarf wird vorgeschlagen, um mögliche Folgeschäden der Pandemie zu begrenzen. Zusatzmaterial online Die Online-Version dieses Beitrags (10.1007/s00278-022-00604-y) enthält die detaillierten Fragen des Fragebogens.
| S-EPMC9243913 | biostudies-literature
Project description:Introduction and objectiveCholesterol homeostasis is a culmination of cellular synthesis, efflux, and catabolism to important physiological entities where short chain fatty acid, butyrate embodied as a key player. This discourse probes the mechanistic molecular details of butyrate action in maintaining host-cholesterol balance.MethodsHepatic mir-122 being the most indispensable regulator of cholesterol metabolic enzymes, we studied upstream players of mir-122 biogenesis in the presence and absence of butyrate in Huh7 cells and mice model. We synthesized unique self-transfecting GMO (guanidinium-morpholino-oligo) linked PMO (Phosphorodiamidate-Morpholino Oligo)-based antisense cell-penetrating reagent to selectively knock down the key player in butyrate mediated cholesterol regulation.ResultsWe showed that butyrate treatment caused upregulation of RNA-binding protein, AUF1 resulting in RNase-III nuclease, Dicer1 instability, and significant diminution of mir-122. We proved the importance of AUF1 and sequential downstream players in AUF1-knock-down mice. Injection of GMO-PMO of AUF1 in mouse caused near absence of AUF1 coupled with increased Dicer1 and mir-122, and reduced serum cholesterol regardless of butyrate treatment indicating that butyrate acts through AUF1.ConclusionThe roster of intracellular players was as follows: AUF1-Dicer1-mir-122 for triggering butyrate driven hypocholesterolemia. To our knowledge this is the first report linking AUF-1 with cholesterol biogenesis.
| S-EPMC9806232 | biostudies-literature
Project description: Not available
| S-EPMC7905125 | biostudies-literature
Project description:Atopic dermatitis (AD) is a chronic pruritic inflammatory skin disease. We recently described an animal model in which repeated epicutaneous applications of a house dust mite extract and Staphylococcal enterotoxin B induced eczematous skin lesions. In this study we showed that global gene expression patterns are very similar between human AD skin and allergen/staphylococcal enterotoxin B–induced mouse skin lesions, particularly in the expression of genes related to epidermal growth/differentiation, skin barrier, lipid/energy metabolism, immune response, or extracellular matrix. In this model, mast cells and T cells, but not B cells or eosinophils, were shown to be required for the full expression of dermatitis, as revealed by reduced skin inflammation and reduced serum IgE levels in mice lacking mast cells or T cells (TCRb-/- or Rag1-/-). The clinical severity of dermatitis correlated with the numbers of mast cells, but not eosinophils. Consistent with the idea that T helper type 2 (Th2) cells play a predominant role in allergic diseases, the receptor for the Th2-promoting cytokine thymic stromal lymphopoietin and the high-affinity IgE receptor, FceRI, were required to attain maximal clinical scores. Therefore, this clinically relevant model provides mechanistic insights into the pathogenic mechanism of human AD.
2013-12-10 | GSE53132 | GEO
Project description:This study aims to evaluate the effect of AUF-1 knokdown on the function and global gene expression in human skin cells. Cells with AUF1 silencing were compared with control cells by RNA-Seq. Cell senescence is a tightly controlled, programmed process. Although the mechanism of cell senescence is unclear, the concept that cell aging is an interaction results between enviomental and genetic factors has been accepted. Changes in the expression of related genes are the genetic basis of cell senescence. AUF1 gene has been proved that can recognize classical mRNA and regulated its expression level. These classical mRNA include those encoding cecle-regulating proteins, apoptotic regulators, cytokines and proto-oncogenes. Our study use overexpression and interference lentivirus of AUF1 to transfect WS1 cell to process cell transcriptome sequencing. Through the Bioinformatics analysis results, we want to see some different expressed genes related to cell cecle, cell apoptosis and so on. Our study can provide basis research to further expound AUF1 gene, play an important role of aging mechanism.
2019-10-10 | GSE138621 | GEO
Project description: Not available
| S-EPMC10946923 | biostudies-literature
Project description:PurposeMeasurement of IgE specific to purified house dust mite (HDM) allergens may improve allergy diagnosis. This study aimed to investigate the sensitization profiles of Korean HDM allergic subjects suffering from respiratory allergy and atopic dermatitis (AD) to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20.MethodsRecombinant HDM allergens were produced in Pichia pastoris (Der f 1) or Escherichia coli (5 allergens). IgE reactivity to the individual recombinant allergens and total extract of mite was assessed by ELISA.ResultsDer f 1 was recognized by 79.1%, Der f 2 by 79.1%, Der f 6 by 9.3%, Der f 8 by 6.2%, Der f 10 by 6.2%, and Der f 20 by 6.6% of the patients' sera tested, while the prevalence of IgE reactivity to total mite extract was 94.7%. Combination of Der f 1 and Der f 2 had a sensitivity of 87.6%. Specific IgE to Der f 2 alone was detected from 89.4% of HDM-sensitized respiratory allergy subjects and 92.3% to the combination of the 2 major allergens Der f 1 and Der f 2. However, sera from fewer patients with AD, namely 72.4% and 71.0%, recognized Der f 1 and Der f 2, respectively. The combination of 2 major allergens allowed diagnosis of 84.5% of the AD patients. No correlation between sensitization to specific allergens and HDM allergy entity was found.ConclusionsDer f 2 was the most frequently sensitized allergen among the HDM-sensitized respiratory and AD patients in Korea, and the combination of the group 1 and 2 major allergens increased the diagnostic sensitivity. Minor allergens did not significantly improve diagnostic sensitivity. However, further studies are needed to analyze the relationship between sensitization to other HDM allergens and the disease entity of the HDM allergy.
| S-EPMC4509661 | biostudies-literature
Project description:Atopic dermatitis (AD) is a chronic pruritic inflammatory skin disease. We recently described an animal model in which repeated epicutaneous applications of a house dust mite extract and Staphylococcal enterotoxin B induced eczematous skin lesions. In this study we showed that global gene expression patterns are very similar between human AD skin and allergen/staphylococcal enterotoxin B–induced mouse skin lesions, particularly in the expression of genes related to epidermal growth/differentiation, skin barrier, lipid/energy metabolism, immune response, or extracellular matrix. In this model, mast cells and T cells, but not B cells or eosinophils, were shown to be required for the full expression of dermatitis, as revealed by reduced skin inflammation and reduced serum IgE levels in mice lacking mast cells or T cells (TCRb-/- or Rag1-/-). The clinical severity of dermatitis correlated with the numbers of mast cells, but not eosinophils. Consistent with the idea that T helper type 2 (Th2) cells play a predominant role in allergic diseases, the receptor for the Th2-promoting cytokine thymic stromal lymphopoietin and the high-affinity IgE receptor, FceRI, were required to attain maximal clinical scores. Therefore, this clinically relevant model provides mechanistic insights into the pathogenic mechanism of human AD. A total of six samples were analyzed. Back skin samples from healthy or AD-induced C57BL/6, PLC-beta 3 KO (C57BL/6 background), and NC/Nga mice were collected for total RNA extraction. Pooled RNA from 2-4 mice per condition were used for analysis.
2013-12-10 | E-GEOD-53132 | biostudies-arrayexpress