Membrane therapy using DHA suppresses epidermal growth factor receptor signaling by disrupting nanocluster formation.

ABSTRACT: Epidermal growth factor receptor (EGFR) signaling drives the formation of many types of cancer, including colon cancer. Docosahexaenoic acid (DHA, 22∶6^{Δ4,7,10,13,16,19}), a chemoprotective long-chain n-3 polyunsaturated fatty acid suppresses EGFR signaling. However, the mechanism underlying this phenotype remains unclear. Therefore, we used super-resolution microscopy techniques to investigate the mechanistic link between EGFR function and DHA-induced alterations to plasma membrane nanodomains. Using isogenic in vitro (YAMC and IMCE mouse colonic cell lines) and in vivo (Drosophila, wild type and Fat-1 mice) models, cellular DHA enrichment via therapeutic nanoparticle delivery, endogenous synthesis, or dietary supplementation reduced EGFR-mediated cell proliferation and downstrea

SUBMITTER: Fuentes NR 

PROVIDER: S-EPMC7933808 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature