Different Cre systems induce differential microRNA landscapes and abnormalities in the female reproductive tracts of Dgcr8 conditional knockout mice.

ABSTRACT:

Objectives

The female reproductive tract comprises several different cell types. Using three representative Cre systems, we comparatively analysed the phenotypes of Dgcr8 conditional knockout (cKO) mice to understand the function of Dgcr8, involved in canonical microRNA biogenesis, in the female reproductive tract.

Materials and methods

Dgcr8^f/f mice were crossed with Ltf^icre/+ , Amhr2^cre/+ or PR^cre/+ mice to produce mice deficient in Dgcr8 in epithelial (Dgcr8^ed/ed ), mesenchymal (Dgcr8^md/md ) and all the compartments (Dgcr8^td/td ) in the female reproductive tract. Reproductive phenotypes were evaluated in Dgcr8 cKO mice. Uteri and/or oviducts were used for small RNA-seq, mRNA-seq, real-time RT-PCR

SUBMITTER: Kim YS 

PROVIDER: S-EPMC7941225 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature