Ontology highlight
ABSTRACT:
SUBMITTER: Maric D
PROVIDER: S-EPMC7946933 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Maric Dragan D Jahanipour Jahandar J Li Xiaoyang Rebecca XR Singh Aditi A Mobiny Aryan A Van Nguyen Hien H Sedlock Andrea A Grama Kedar K Roysam Badrinath B
Nature communications 20210310 1
Mapping biological processes in brain tissues requires piecing together numerous histological observations of multiple tissue samples. We present a direct method that generates readouts for a comprehensive panel of biomarkers from serial whole-brain slices, characterizing all major brain cell types, at scales ranging from subcellular compartments, individual cells, local multi-cellular niches, to whole-brain regions from each slice. We use iterative cycles of optimized 10-plex immunostaining wit ...[more]