In-Depth Characterization of the Staphylococcus aureus Phosphoproteome Reveals New Targets of Stk1.

ABSTRACT: Staphylococcus aureus is a major cause of infections worldwide, and infection results in a variety of diseases. As of no surprise, protein phosphorylation is an important game player in signaling cascades and has been shown to be involved in S. aureus virulence. Albeit long neglected, eukaryotic-type serine/threonine kinases in S. aureus have been implicated in this complex signaling cascades. Due to the substoichiometric nature of protein phosphorylation and a lack of suitable analysis tools, the knowledge of these cascades is, however, to date, still limited. Here, were apply an optimized protocol for efficient phosphopeptide enrichment via Fe³⁺-IMAC followed by LC-MS/MS to get a better understanding of the impact of protein phosphorylation on the complex signaling networks in

SUBMITTER: Prust N 

PROVIDER: S-EPMC7950182 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature